Article
Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays.
Molecular ecology resources - 1 May 2017
Cruz Vanessa P, Vera Manuel, Pardo Belén G, Taggart John, Martinez Paulino, Oliveira Claudio, Foresti Fausto
Abstract excerpt
Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226 774 reads (65.5 Mb) were obtained (mean read length 289 ± 183 bp) detecting a total of 5399 contigs (mean contig length: 396 ± 91 bp). Mining this data set, a...
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