Article
Generation of KCL040 clinical grade human embryonic stem cell line.
Stem cell research - 1 Jan 2016
Jacquet Laureen, Wood Victoria, Kadeva Neli, Cornwell Glenda, Codognotto Stefano, Stephenson Emma, Ilic Dusko
Abstract excerpt
The KCL040 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation...
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