Article
Standardization and application of the tetraprimer ARMS-PCR technique for screening of the E198A SNP in the β-tubulin gene of hookworm populations in Brazil.
Veterinary parasitology - 15 Jul 2016
Furtado Luis Fernando Viana, Alves William Pereira, Moreira Thayse Batista, Costa Junior Livio Martins, Miranda Rodrigo Rodrigues Cambraia, Rabelo Élida Mara Leite
Abstract excerpt
The tetraprimer ARMS-PCR technique is efficient for SNP detection and can be used to search for polymorphisms associated with drug resistance. However, the establishment of this methodology is not always straightforward because of the constraints on primer design due to the restrictions of the polymorphic regions. Here, we describe the standardization of the tetraprimer ARMS-PCR methodology for the detection of a...
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