Article
CRISPR/Cas9-Derived Mutations Both Inhibit HIV-1 Replication and Accelerate Viral Escape.
Cell reports - 19 Apr 2016
Wang Zhen, Pan Qinghua, Gendron Patrick, Zhu Weijun, Guo Fei, Cen Shan, Wainberg Mark A, Liang Chen
Abstract excerpt
Cas9 cleaves specific DNA sequences with the assistance of a programmable single guide RNA (sgRNA). Repairing this broken DNA by the cell's error-prone non-homologous end joining (NHEJ) machinery leads to insertions and deletions (indels) that often impair DNA function. Using HIV-1, we have now demonstrated that many of these indels are indeed lethal for the virus, but that others lead to the emergence of...
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