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Article

Transfer of the cytochrome P450-dependent dhurrin pathway from Sorghum bicolor into Nicotiana tabacum chloroplasts for light-driven synthesis.

Journal of experimental botany - 1 Apr 2016

Gnanasekaran Thiyagarajan, Karcher Daniel, Nielsen Agnieszka Zygadlo, Martens Helle Juel, Ruf Stephanie, Kroop Xenia, Olsen Carl Erik, Motawie Mohammed Saddik, Pribil Mathias, Møller Birger Lindberg, Bock Ralph, Jensen Poul Erik

Abstract excerpt

Plant chloroplasts are light-driven cell factories that have great potential to act as a chassis for metabolic engineering applications. Using plant chloroplasts, we demonstrate how photosynthetic reducing power can drive a metabolic pathway to synthesise a bio-active natural product. For this purpose, we stably engineered the dhurrin pathway from Sorghum bicolor into the chloroplasts of Nicotiana tabacum...

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Identifiers

PMID: 26969746
DOI: 10.1093/jxb/erw067
PMCID: PMC4809297

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