Article
An Inducible Retroviral Expression System for Tandem Affinity Purification Mass-Spectrometry-Based Proteomics Identifies Mixed Lineage Kinase Domain-like Protein (MLKL) as an Heat Shock Protein 90 (HSP90) Client.
Molecular & cellular proteomics : MCP - 1 Mar 2016
Bigenzahn Johannes W, Fauster Astrid, Rebsamen Manuele, Kandasamy Richard K, Scorzoni Stefania, Vladimer Gregory I, Müller André C, Gstaiger Matthias, Zuber Johannes, Bennett Keiryn L, Superti-Furga Giulio
Abstract excerpt
Tandem affinity purification-mass spectrometry (TAP-MS) is a popular strategy for the identification of protein-protein interactions, characterization of protein complexes, and entire networks. Its employment in cellular settings best fitting the relevant physiology is limited by convenient expression vector systems. We developed an easy-to-handle, inducible, dually selectable retroviral expression vector...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.