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Article

The unusual metal ion binding ability of histidyl tags and their mutated derivatives.

Dalton transactions (Cambridge, England : 2003) - 7 Apr 2016

Brasili Davide, Watly Joanna, Simonovsky Eyal, Guerrini Remo, Barbosa Nuno A, Wieczorek Robert, Remelli Maurizio, Kozlowski Henryk, Miller Yifat

Abstract excerpt

Polyhistidine-tags are often used for the affinity purification of polyhistidine-tagged recombinant proteins. These sequences are also found in nature and are often highly conserved across different species. However, their exact role in the biological systems is not clear. The purpose of this work is to shed light on the behavior of poly-His sequences in their interactions with metal ions. This work illustrates...

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PMID: 26923149
DOI: 10.1039/c5dt04747a

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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