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Article

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

7 Jan 2016

Abstract excerpt

This protocol describes a simple and quick method to isolate and characterize mouse antral GV (Germinal Vesicle) oocytes as able (SN, Surrounded Nucleolus) or unable (NSN, Not Surrounded Nucleolus) to develop to the blastocyst stage after in vitro maturation (IVM) and in vitro fertilization (IVF). It makes use of Hoeschst33342 (or any other DNA intercalating dye) able to bind to the heterochromatin of the...

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PMID: 26780158
DOI: 10.3791/53616

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