Article
BRCA somatic and germline mutation detection in paraffin embedded ovarian cancers by next-generation sequencing.
Oncotarget - 12 Jan 2016
Mafficini Andrea, Simbolo Michele, Parisi Alice, Rusev Borislav, Luchini Claudio, Cataldo Ivana, Piazzola Elena, Sperandio Nicola, Turri Giona, Franchi Massimo, Tortora Giampaolo, Bovo Chiara, Lawlor Rita T, Scarpa Aldo
Abstract excerpt
BRCA mutated ovarian cancers respond better to platinum-based therapy and to the recently approved PARP-inhibitors. There is the need for efficient and timely methods to detect both somatic and germline mutations using formalin-fixed paraffin-embedded (FFPE) tissues and commercially available technology. We used a commercial kit exploring all exons and 50bp exon-intron junctions of BRCA1 and BRCA2 genes, and...
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