Article
Ultrafast Assessment of the Presence of a High-Risk Mycobacterium tuberculosis Strain in a Population.
Journal of clinical microbiology - 1 Mar 2016
Pérez-Lago Laura, Herranz Marta, Comas Iñaki, Ruiz-Serrano María Jesús, López Roa Paula, Bouza Emilio, García-de-Viedma Darío
Abstract excerpt
A persistent 8-year infection by a Beijing Mycobacterium tuberculosis strain from a previous outbreak after importation from West Africa obliged us to investigate secondary cases. We developed a multiplex PCR method based on whole-genome sequencing to target strain-specific single nucleotide polymorphisms (SNPs). In 1 week, we analyzed 868 isolates stored over 6 years. Only 2 cases (immigrants from Guinea...
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