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Article

Establishment of pancreatic cancer stem cells by flow cytometry and their biological characteristics.

International journal of clinical and experimental pathology - 1 Jan 2015

Li Dandan, Su Dongyue, Xue Lei, Liu Yang, Pang Wuyan

Abstract excerpt

To investigate the method of separating human pancreatic cancer stem cells by Hoechst 33342 labeled flow cytometry and to analyze the biological properties of pancreatic cancer stem cells. The human pancreatic cancer cell line PC-3 was divided into SP and non-SP cells by flow cytometry. The number of two cell clone spheres and nude mice tumor formation rates were compared by cultivating in serum-free medium; The...

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Identifiers

PMID: 26617845
PMCID: PMC4637660

Genes

ABCA2
ABCG2

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