Article
A silent allele in the locus D5S818 contained within the PowerPlex®21 PCR Amplification Kit.
Legal medicine (Tokyo, Japan) - 1 Nov 2015
Chen Ling, Tai Yunchun, Qiu Pingming, Du Weian, Liu Chao
Abstract excerpt
Three paternity tests cases were found with a single locus mismatch at the locus D5S818 with PowerPlex®21 PCR Amplification Kit (Promega). Forward and reverse primers were redesigned to type the samples again and to evaluate if there were alleles dropped out. The results showed the existence of a silent allele 12 in all the three families, due to a point mutation that changed cytosine to adenine at 90 nucleotides...
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