Article
A silent allele in the locus D19S433 contained within the AmpFlSTR Identifiler PCR Amplification Kit.
Legal medicine (Tokyo, Japan) - 1 Mar 2010
Tsuji Akiko, Ishiko Atsushi, Umehara Takahiro, Usumoto Yosuke, Hikiji Wakako, Kudo Keiko, Ikeda Noriaki
Abstract excerpt
We present two cases where a single locus mismatch was found in the locus D19S433 using the AmpFlSTR Identifiler PCR Amplification Kit (Applied Biosystems) (Identifiler Kit) during paternity and maternity tests. This mismatch differed from the mismatch pattern where there is usually a one repeat difference. We designed forward and reverse primers so that they were positioned further away from the primer set...
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