Article
Identification and characterization of the zosA gene involved in copper uptake in Bacillus subtilis 168.
Bioscience, biotechnology, and biochemistry - 1 Jan 2016
Fukuhara Takahiro, Kobayashi Kazuo, Kanayama Yousuke, Enomoto Shu-ichi, Kondo Taeko, Tsunekawa Naoki, Nemoto Michiko, Ogasawara Naotake, Inagaki Kenji, Tamura Takashi
Abstract excerpt
DL-Penicillamine, a copper-specific metal chelator, remarkably suppressed the growth of Bacillus subtilis 168 when added to a synthetic medium under Cu(2+) limitation. DNA microarray and screening of 2,602 knockout mutants showed that the zosA gene was de-repressed in the presence of 0.1% dl-penicillamine, and that the zosA mutant was sensitive to dl-penicillamine medium. The zosA mutant delayed the growth under...
Topics
- Bacillus subtilis
- Copper
- Genes, Bacterial
- Mutation
- Oligonucleotide Array Sequence Analysis