Article
Synthesis of vanillic acid using whole cell nitrilase of wild and mutant Gordonia terrae.
Bioprocess and biosystems engineering - 1 Jan 2016
Bhalla Tek Chand, Prashant, Kumari Nisha, Kumar Vijay, Kumar Virender, Savitri
Abstract excerpt
The resting cells of Gordonia terrae mutant E9 having enhanced nitrilase activity were used for biotransformation of 4-hydroxy-3-methoxybenzonitrile into vanillic acid. The maximum conversion was observed in 0.1 M phosphate buffer (pH 8.0), using 60 mM substrate and 0.75 mgDCW resting cells in 1 mL reaction at 40 °C. Km of the whole cell nitrilase of wild and mutant strains of G. terrae for this substrate were 20...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.