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Evaluation of Automatic Analysis of Ultradeep Pyrosequencing Raw Data to Determine Percentages of HIV Resistance Mutations in Patients Followed-Up in Hospital.

AIDS research and human retroviruses - 1 Jan 2016

Bellecave Pantxika, Recordon-Pinson Patricia, Fleury Hervé

Abstract excerpt

A major obstacle to using next generation sequencing (NGS) technology in clinical routine practice is reliable data analysis. Thousands of sequences need to be aligned and validated, to exclude sequencing artifacts and generate accurate results. We compared two analysis pipelines for Roche 454 ultradeep pyrosequencing (UDPS) raw data generated from HIV-1 clinical samples: a commercial and fully automated...

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PMID: 26529549
DOI: 10.1089/AID.2015.0201

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