Article
Kinetic consequences of introducing a proximal selenocysteine ligand into cytochrome P450cam.
Biochemistry - 10 Nov 2015
Vandemeulebroucke An, Aldag Caroline, Stiebritz Martin T, Reiher Markus, Hilvert Donald
Abstract excerpt
The structural, electronic, and catalytic properties of cytochrome P450cam are subtly altered when the cysteine that coordinates to the heme iron is replaced with a selenocysteine. To map the effects of the sulfur-to-selenium substitution on the individual steps of the catalytic cycle, we conducted a comparative kinetic analysis of the selenoenzyme and its cysteine counterpart. Our results show that the more...
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