Article
Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes.
Stem cells translational medicine - 1 Feb 2017
Lin Yongshun, Linask Kaari L, Mallon Barbara, Johnson Kory, Klein Michael, Beers Jeanette, Xie Wen, Du Yubin, Liu Chengyu, Lai Yinzhi, Zou Jizhong, Haigney Mark, Yang Hushan, Rao Mahendra, Chen Guokai
Abstract excerpt
Cardiomyocytes can be differentiated from human pluripotent stem cells (hPSCs) in defined conditions, but efficient and consistent cardiomyocyte differentiation often requires expensive reagents such as B27 supplement or recombinant albumin. Using a chemically defined albumin-free (E8 basal) medium, we identified heparin as a novel factor that significantly promotes cardiomyocyte differentiation efficiency, and...
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