Article
The fibronectin-binding protein EfbA contributes to pathogenesis and protects against infective endocarditis caused by Enterococcus faecalis.
Infection and immunity - 1 Dec 2015
Singh Kavindra V, La Rosa Sabina Leanti, Somarajan Sudha R, Roh Jung Hyeob, Murray Barbara E
Abstract excerpt
EfbA is a PavA-like fibronectin adhesin of Enterococcus faecalis previously shown to be important in experimental urinary tract infection. Here, we expressed and purified the E. faecalis OG1RF EfbA and confirmed that this protein binds with high affinity to immobilized fibronectin, collagen I, and collagen V. We constructed an efbA deletion mutant and demonstrated that its virulence was significantly attenuated...
Topics
- Adhesins, Bacterial
- Animals
- Binding Sites
- Biofilms
- Collagen Type I
- Collagen Type V
- Endocarditis, Bacterial
- Enterococcus faecalis
- Escherichia coli
- Fibronectins
- Gene Expression
- Genetic Complementation Test
- Gram-Positive Bacterial Infections