Article
Mutation Scanning in Wheat by Exon Capture and Next-Generation Sequencing.
PloS one - 1 Jan 2015
King Robert, Bird Nicholas, Ramirez-Gonzalez Ricardo, Coghill Jane A, Patil Archana, Hassani-Pak Keywan, Uauy Cristobal, Phillips Andrew L
Abstract excerpt
Targeted Induced Local Lesions in Genomes (TILLING) is a reverse genetics approach to identify novel sequence variation in genomes, with the aims of investigating gene function and/or developing useful alleles for breeding. Despite recent advances in wheat genomics, most current TILLING methods are low to medium in throughput, being based on PCR amplification of the target genes. We performed a pilot-scale...
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