Article
High-throughput and quantitative genome-wide messenger RNA sequencing for molecular phenotyping.
BMC genomics - 5 Aug 2015
Collins John E, Wali Neha, Sealy Ian M, Morris James A, White Richard J, Leonard Steven R, Jackson David K, Jones Matthew C, Smerdon Nathalie C, Zamora Jorge, Dooley Christopher M, Carruthers Samantha N, Barrett Jeffrey C, Stemple Derek L, Busch-Nentwich Elisabeth M
Abstract excerpt
BACKGROUND: We present a genome-wide messenger RNA (mRNA) sequencing technique that converts small amounts of RNA from many samples into molecular phenotypes. It encompasses all steps from sample preparation to sequence analysis and is applicable to baseline profiling or perturbation measurements. RESULTS: Multiplex sequencing of transcript 3' ends identifies differential transcript abundance independent of gene...
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