Article
Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells.
Nature cell biology - 1 Aug 2015
Patsch Christoph, Challet-Meylan Ludivine, Thoma Eva C, Urich Eduard, Heckel Tobias, O'Sullivan John F, Grainger Stephanie J, Kapp Friedrich G, Sun Lin, Christensen Klaus, Xia Yulei, Florido Mary H C, He Wei, Pan Wei, Prummer Michael, Warren Curtis R, Jakob-Roetne Roland, Certa Ulrich, Jagasia Ravi, Freskgård Per-Ola, Adatto Isaac, Kling Dorothee, Huang Paul, Zon Leonard I, Chaikof Elliot L, Gerszten Robert E, Graf Martin, Iacone Roberto, Cowan Chad A
Abstract excerpt
The use of human pluripotent stem cells for in vitro disease modelling and clinical applications requires protocols that convert these cells into relevant adult cell types. Here, we report the rapid and efficient differentiation of human pluripotent stem cells into vascular endothelial and smooth muscle cells. We found that GSK3 inhibition and BMP4 treatment rapidly committed pluripotent cells to a mesodermal...
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