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Article

Phenotypes of trypsin- and collagenase-prepared bovine corneal endothelial cells in the presence of a selective Rho kinase inhibitor, Y-27632.

Molecular vision - 1 Jan 2015

Su Chien-Chia, Chen Chun-Wen, Ho Wei-Ting, Hu Fung-Rong, Lee Shwu-Huey, Wang I-Jong

Abstract excerpt

PURPOSE: To optimize isolation of viable bovine corneal endothelial cells (BCECs), we evaluated the effectiveness of various preparation protocols. This entailed comparing the effects of collagenase A and trypsin in the presence and absence of a Rho kinase inhibitor, Y-27632, on proliferation and tight junctional and cytoskeletal integrity during their expansion. METHODS: 5-bromo-2'-deoxyuridine (BrdU)...

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Identifiers

PMID: 26097378
PMCID: PMC4455892

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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