Article
Bioinformatic Amplicon Read Processing Strategies Strongly Affect Eukaryotic Diversity and the Taxonomic Composition of Communities.
PloS one - 1 Jan 2015
Majaneva Markus, Hyytiäinen Kirsi, Varvio Sirkka Liisa, Nagai Satoshi, Blomster Jaanika
Abstract excerpt
Amplicon read sequencing has revolutionized the field of microbial diversity studies. The technique has been developed for bacterial assemblages and has undergone rigorous testing with mock communities. However, due to the great complexity of eukaryotes and the numbers of different rDNA copies, analyzing eukaryotic diversity is more demanding than analyzing bacterial or mock communities, so studies are needed...
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