Article
Analysis of antibody clonotype by affinity immunoblotting.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Kurien Biji T, Scofield R Hal
Abstract excerpt
A sensitive and specific method to analyze specific antibody clonotype changes in a lupus patient who developed autoantibodies to the Ro 60 autoantigen under observation is described. Patient sera, collected over several years, were separated by flatbed isoelectric focusing (IEF) and analyzed by affinity immunoblotting utilizing Ro 60-coated nitrocellulose membrane. When the Ro 60-coated nitrocellulose was laid...
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