Article
PCR with sequence-specific primers for typing of diallelic blood groups.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Rink Gabriele, Scharberg Erwin A, Bugert Peter
Abstract excerpt
PCR with sequence-specific primers (PCR-SSP) is a cost-effective and robust method for the analysis of single nucleotide polymorphisms (SNPs). Many blood group antigens and the antithetic antigens are based on a diallelic SNP in the coding region of the corresponding blood group gene. Here, we describe PCR-SSP protocols for genotyping 24 blood group antigens based on 12 diallelic SNPs. We also provide protocols...
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