Posttranslational Modifications of Intact Proteins Detected by NMR Spectroscopy: Application to Glycosylation

Abstract excerpt

Posttranslational modifications (PTMs) are an integral part of the majority of proteins. The characterization of structure and function of PTMs can be very challenging especially for glycans. Existing methods to analyze PTMs require complicated sample preparations and suffer from missing certain modifications, the inability to identify linkage types and thus chemical structure. We present a direct, robust, and...