Article
Mutagenicity monitoring following battlefield exposures: Molecular analysis of HPRT mutations in Gulf War I veterans exposed to depleted uranium.
Environmental and molecular mutagenesis - 1 Aug 2015
Nicklas Janice A, Albertini Richard J, Vacek Pamela M, Ardell Stephanie K, Carter Elizabeth W, McDiarmid Melissa A, Engelhardt Susan M, Gucer Patricia W, Squibb Katherine S
Abstract excerpt
Molecular studies that involved cDNA and genomic DNA sequencing as well as multiplex PCR of the HPRT gene were performed to determine the molecular mutational spectrum for 1,377 HPRT mutant isolates obtained from 61 Veterans of the 1991 Gulf War, most of whom were exposed to depleted uranium (DU). Mutant colonies were isolated from one to four times from each Veteran (in 2003, 2005, 2007, and/or 2009). The...
Topics
- Amino Acid Sequence
- Base Sequence
- Cells, Cultured
- DNA Mutational Analysis
- Gene Frequency
- Gulf War
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Longitudinal Studies
- Male
- Military Personnel
- Molecular Sequence Data