Article
Temperature-Sensitive Mutants in the Influenza A Virus RNA Polymerase: Alterations in the PA Linker Reduce Nuclear Targeting of the PB1-PA Dimer and Result in Viral Attenuation.
Journal of virology - 1 Jun 2015
Da Costa Bruno, Sausset Alix, Munier Sandie, Ghounaris Alexandre, Naffakh Nadia, Le Goffic Ronan, Delmas Bernard
Abstract excerpt
UNLABELLED: The influenza virus RNA-dependent RNA polymerase catalyzes genome replication and transcription within the cell nucleus. Efficient nuclear import and assembly of the polymerase subunits PB1, PB2, and PA are critical steps in the virus life cycle. We investigated the structure and func...
Topics
- Active Transport, Cell Nucleus
- Animals
- Circular Dichroism
- Female
- Genetic Complementation Test
- Humans
- Influenza A Virus, H1N1 Subtype
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mutant Proteins
- Mutation
- Protein Binding
- Protein Interaction Domains and Motifs