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Article

BMP2 Repression and Optimized Culture Conditions Promote Human Bone Marrow-Derived Mesenchymal Stem Cell Isolation

1 Mar 2015

Abstract excerpt

AIM: Human mesenchymal stem cells (hMSC) are multipotent progenitor cells. We propose the optimization of hMSC isolation and recovery using the application of a controlled hypoxic environment. MATERIALS & METHODS: We evaluated oxygen, glucose and serum in the recovery of hMSC from bone marrow (BMhMSC). Colony forming units-fibroblastic, cell numbers, tri-lineage differentiation, immunofluorescence and microarray...

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PMID: 25835477
DOI: 10.2217/rme.14.67

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