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Human mesenchymal stem cell culture: rapid and efficient isolation and expansion in a defined serum-free medium

11 Jul 2011

Abstract excerpt

Human mesenchymal stem cells (hMSCs) are typically obtained for research or therapeutic applications by isolating and subculturing adherent cells from bone marrow on tissue-culture substrates using growth media. The purity and properties of the resulting populations can be affected greatly by the conditions under which they are cultured. Fetal bovine serum (FBS), although ill-defined, has been widely used as a...

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PMID: 21744510
DOI: 10.1002/term.441

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