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Quantification of female and male Plasmodium falciparum gametocytes by reverse transcriptase quantitative PCR

1 Jan 2015

Abstract excerpt

The transmission of malaria parasites depends on the presence of sexual stages (gametocytes) in the blood, making the ratio and densities of female and male gametocytes important determinants of parasite fitness. This manuscript describes the development of reverse transcriptase quantitative PCR (RT-qPCR) assays to separately quantify mature female and male gametocytes of the human malaria parasite Plasmodium...

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PMID: 25827756
DOI: 10.1016/j.molbiopara.2015.03.006

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