Article
Rapid genotyping of Plasmodium vivax Pvs25 and Pv38 genes by using mismatch specific endonuclease.
Tropical biomedicine - 1 Dec 2014
Jang J W, Cho C H, Kim J Y, Koh Y E, Woo M K, Kim K A, Yoon S Y, Lim M S, Han E T, An S S A, Lim C S
Abstract excerpt
Mismatch specific endonuclease (MSE) method was used to detect natural polymorphisms in Pvs25 and Pv38 genes of Plasmodium vivax. Eighty seven patients with P. vivax were recruited in the Republic of Korea (ROK). Pvs25 and Pv38 genes were amplified by polymerase chain reaction (PCR), and the PCR amplicons were mixed with reference DNA sequences. Following the denaturation and gradual annealing, the product...
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