Article
Real-time PCR genotyping assay for feline erythrocyte pyruvate kinase deficiency and mutant allele frequency in purebred cats in Japan.
The Journal of veterinary medical science - 1 Jun 2015
Kushida Kazuya, Giger Urs, Tsutsui Toshihiko, Inaba Megumi, Konno Yoshio, Hayashi Kureha, Noguchi Kana, Yabuki Akira, Mizukami Keijiro, Kohyama Moeko, Endo Yasuyuki, Yamato Osamu
Abstract excerpt
Erythrocyte pyruvate kinase (PK) deficiency is an inherited glycolytic erythroenzymopathy caused by mutations of the PKLR gene. A causative mutation of the feline PKLR gene was originally identified in Abyssinian and Somali cats in the U.S.A. In the present study, a TaqMan probe-based real-time PCR genotyping assay was developed and evaluated for rapid genotyping and large-scale screening for this mutation....
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