Article
Real-time PCR genotyping assay for canine progressive rod-cone degeneration and mutant allele frequency in Toy Poodles, Chihuahuas and Miniature Dachshunds in Japan.
The Journal of veterinary medical science - 1 Mar 2016
Kohyama Moeko, Tada Naomi, Mitsui Hiroko, Tomioka Hitomi, Tsutsui Toshihiko, Yabuki Akira, Rahman Mohammad Mahbubur, Kushida Kazuya, Mizukami Keijiro, Yamato Osamu
Abstract excerpt
Canine progressive rod-cone degeneration (PRCD) is a middle- to late-onset, autosomal recessive, inherited retinal disorder caused by a substitution (c.5G>A) in the canine PRCD gene that has been identified in 29 or more purebred dogs. In the present study, a TaqMan probe-based real-time PCR assay was developed and evaluated for rapid genotyping and large-scale screening of the mutation. Furthermore, a genotyping...
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