Article
An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii.
The Plant cell - 1 Feb 2016
Li Xiaobo, Zhang Ru, Patena Weronika, Gang Spencer S, Blum Sean R, Ivanova Nina, Yue Rebecca, Robertson Jacob M, Lefebvre Paul A, Fitz-Gibbon Sorel T, Grossman Arthur R, Jonikas Martin C
Abstract excerpt
The green alga Chlamydomonas reinhardtii is a leading unicellular model for dissecting biological processes in photosynthetic eukaryotes. However, its usefulness has been limited by difficulties in obtaining mutants in specific genes of interest. To allow generation of large numbers of mapped mutants, we developed high-throughput methods that (1) enable easy maintenance of tens of thousands of Chlamydomonas...
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