Article
Driving Human Granulosa-Luteal Cells Recovered From In Vitro Fertilization Cycles Toward the Follicular Phase Phenotype.
Reproductive sciences (Thousand Oaks, Calif.) - 1 Aug 2015
Vireque Alessandra Aparecida, Campos Jacira Ribeiro, Dentillo Daniel Blasioli, Bernuci Marcelo Picinin, Campos Carolina Oliveira, Silva-de-Sá Marcos Felipe, Ferriani Rui Alberto, Nunes Altacílio Aparecido, Rosa-e-Silva Ana Carolina Japur de Sá
Abstract excerpt
Culture systems are available for human granulosa cells (GCs) that perpetuate luteinization. The present study examines the plating density effects and long-term serum-free culture on the in vitro dynamics differentiation of luteinizing human GCs. Cells were cultured in serum-free α-minimum essential medium (α-MEM) or serum-based tissue culture medium (TCM). The time course of GCs morphology and secretion of...
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