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Article

Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by addition of follicular fluid.

Journal of assisted reproduction and genetics - 1 Sept 2011

Bruckova Lenka, Soukup Tomas, Visek Benjamin, Moos Jiri, Moosova Martina, Pavelkova Jana, Rezabek Karel, Kucerova Lenka, Micuda Stanislav, Brcakova Eva, Mokry Jaroslav

Abstract excerpt

PURPOSE: The aim of this study was to develop and optimize a strategy for long-term cultivation of luteinizing human granulosa cells (GCs). METHODS: GCs were cultivated in DMEM/F12 medium supplemented with 2% fetal calf serum. In vitro proliferation of GCs was supported by follicular fluid as well as FSH and growth factors. RESULTS: The cultured GCs were maintained for 45 days with a doubling time of 159 ± 24 h....

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Identifiers

PMID: 21822582
DOI: 10.1007/s10815-011-9617-6
PMCID: PMC3220434

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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