RNA-seq analysis identifies new genes regulated by the histone-like nucleoid structuring protein (H-NS) affecting Vibrio cholerae virulence, stress response and chemotaxis.

Abstract excerpt

The histone-like nucleoid structuring protein (H-NS) functions as a transcriptional silencer by binding to AT-rich sequences at bacterial promoters. However, H-NS repression can be counteracted by other transcription factors in response to environmental changes. The identification of potential toxic factors, the expression of which is prevented by H-NS could facilitate the discovery of new regulatory proteins...

Topics

• Bacterial Proteins
• Chemotaxis
• Cholera
• DNA-Binding Proteins
• Gene Deletion
• Gene Expression Profiling
• Gene Expression Regulation, Bacterial
• High-Throughput Nucleotide Sequencing
• Indoles
• Mutation
• Promoter Regions, Genetic
• Protein Binding
• Quorum Sensing