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Enrichment or depletion? The impact of stool pretreatment on metaproteomic characterization of the human gut microbiota

12 Feb 2015

Abstract excerpt

To date, most metaproteomic studies of the gut microbiota employ stool sample pretreatment methods to enrich for microbial components. However, a specific investigation aimed at assessing if, how, and to what extent this may impact on the final taxonomic and functional results is still lacking. Here, stool replicates were either pretreated by differential centrifugation (DC) or not centrifuged. Protein extracts...

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PMID: 25677681
DOI: 10.1002/pmic.201400573

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