Article
Next-generation sequencing is a robust strategy for the high-throughput detection of zygosity in transgenic maize.
Transgenic research - 1 Aug 2015
Fritsch Leonie, Fischer Rainer, Wambach Christoph, Dudek Max, Schillberg Stefan, Schröper Florian
Abstract excerpt
Simple and reliable, high-throughput techniques to detect the zygosity of transgenic events in plants are valuable for biotechnology and plant breeding companies seeking robust genotyping data for the assessment of new lines and the monitoring of breeding programs. We show that next-generation sequencing (NGS) applied to short PCR products spanning the transgene integration site provides accurate zygosity data...
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