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Article

Quantifying mitochondrial content in living cells.

Methods in cell biology - 1 Jan 2015

Viana Matheus Palhares, Lim Swee, Rafelski Susanne M

Abstract excerpt

We describe a novel version of MitoGraph, our fully automated image processing method and software, dedicated to calculating the volume of 3D intracellular structures and organelles in live cells. MitoGraph is optimized and validated for quantifying the volume of tubular mitochondrial networks in budding yeast. We therefore include the experimental protocol, microscopy conditions, and software parameters focusing...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25640425
DOI: 10.1016/bs.mcb.2014.10.003

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