Article
Detection of the genetic variation of polygalacturonase-inhibiting protein gene 2 in autotetraploid alfalfa (Medicago sativa) using an improved SSCP technique.
Genetics and molecular research : GMR - 4 Dec 2014
Gui Z, Liu H Q, Wang Y, Yuan Q H, Xin N, Zhang X, Li X L, Pi Y S, Gao J M
Abstract excerpt
In this study, 2 approaches were adopted to obtain good single-strand conformation polymorphism (SSCP) data for autotetraploid alfalfa; primers were added to PCR products, and fluorescent-labeled primers were utilized. PCR-SSCP conditions for a 331-bp fragment in the coding region of polygalacturonase-inhibiting protein gene 2 in alfalfa (MsPGIP2) were optimized, and the results showed that the best SSCP gel...
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