Article
Development and characterization of simple sequence repeat (SSR) markers based on RNA-sequencing of Medicago sativa and in silico mapping onto the M. truncatula genome.
PloS one - 1 Jan 2014
Wang Zan, Yu Guohui, Shi Binbin, Wang Xuemin, Qiang Haiping, Gao Hongwen
Abstract excerpt
Sufficient codominant genetic markers are needed for various genetic investigations in alfalfa since the species is an outcrossing autotetraploid. With the newly developed next generation sequencing technology, a large amount of transcribed sequences of alfalfa have been generated and are available for identifying SSR markers by data mining. A total of 54,278 alfalfa non-redundant unigenes were assembled through...
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