Article
[Physical-chemical properties of the mutant (protein) form of D-glucose/D-galactose-binding protein GGBP/H152C with an attached fluorescent dye BADAN].
Tsitologiia - 1 Jan 2013
Fonin A V, Stepanenko O V, Povarova O I, Volova E A, Filippova E M, Bublikov G S, Kuznetsova I M, Demchenko A P, Turoverov K K
Abstract excerpt
The influence of various factors on the physico-chemical characteristics and complexation of glucose with a mutant form of D-glucose/D-galactose-binding protein which can be regarded as a sensor of the glucometer, namely the protein GGBP/H152C with solvatochromic dye BADAN attached to the cysteine residue Cys 152, has been investigated. The point mutation His 152Cys and attaching BADAN reduced the affinity of the...
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