Article
Characterization of the minimal length of functional SecA in Escherichia coli.
Biochemical and biophysical research communications - 2 Jan 2015
Na Bing, You Zhipeng, Yang Hsiuchin, Tai Phang C
Abstract excerpt
Previous studies showed that certain regions of E. coli SecA can be deleted from its N- and/or C-termini to complement a SecA amber ts mutant. In this study, we determined and characterized the dispensability of both ends of SecA molecules. With N-terminal intact or 9-aa deleted, 826aa (SecA1-826 and SecA10-826, respectively) is the minimum for complementation activity, while with N-terminus deleted by 2-21aa,...
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