Article
Association of IL8 -105G/A with mastitis somatic cell score in Chinese Holstein dairy cows.
Animal biotechnology - 1 Jan 2015
Chen Renjin, Wang Zhenzhen, Yang Zhangping, Zhu Xiaorong, Ji Dejun, Mao Yongjiang
Abstract excerpt
The single nucleotide polymorphisms (SNPs) in the 5' upstream of bovine IL8 gene were investigated in 810 Chinese Holstein cows from 35 bull families in a dairy farm in Shanghai using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique. The Real-time PCR and Western blot were used to detect the mRNA and protein levels of genotype Chinese Holstein dairy cows. The results showed...
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