Article
High-throughput genotyping with the GoldenGate assay in the complex genome of soybean.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 May 2008
Hyten David L, Song Qijian, Choi Ik-Young, Yoon Mun-Sup, Specht James E, Matukumalli Lakshmi K, Nelson Randall L, Shoemaker Randy C, Young Nevin D, Cregan Perry B
Abstract excerpt
Large numbers of single nucleotide polymorphism (SNP) markers are now available for a number of crop species. However, the high-throughput methods for multiplexing SNP assays are untested in complex genomes, such as soybean, that have a high proportion of paralogous genes. The Illumina GoldenGate assay is capable of multiplexing from 96 to 1,536 SNPs in a single reaction over a 3-day period. We tested the...
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