Article
Quantitative SNP genotyping of polyploids with MassARRAY and other platforms.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Mollinari Marcelo, Serang Oliver
Abstract excerpt
Accurate genotyping is essential for building genetic maps and performing genome assembly of polyploid species. Recent high-throughput techniques, such as Illumina GoldenGate™ and Sequenom iPLEX MassARRAY®, have made it possible to accurately estimate the relative abundances of different alleles even when the ploidy of the population is unknown. Here we describe the experimental methods for collecting these...
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