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Comparing Whole-Genome Sequencing with Sanger Sequencing for <i>spa</i> Typing of Methicillin-Resistant Staphylococcus aureus

9 Oct 2014

Abstract excerpt

spa typing of methicillin-resistant Staphylococcus aureus (MRSA) has traditionally been done by PCR amplification and Sanger sequencing of the spa repeat region. At Hvidovre Hospital, Denmark, whole-genome sequencing (WGS) of all MRSA isolates has been performed routinely since January 2013, and an in-house analysis pipeline determines the spa types. Due to national surveillance, all MRSA isolates are sent to...

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PMID: 25297335
DOI: 10.1128/jcm.01979-14

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